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Human LC-SPIK ELISA Kit - Seravue®
Human LC-SPIK ELISA Kit - Seravue®
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The Seravue® LC-SPIK ELISA Kit is a sensitive enzyme-linked immunosorbent assay designed for the precise quantification of human LC-SPIK levels in serum and plasma samples. It enables accurate in vitro measurement of Liver Cancer–secreted serine protease inhibitor Kazal (LC-SPIK) levels in human biological fluids. This investigational use only (IUO) kit is intended for product evaluation and performance assessment in detecting LC-SPIK concentrations but is not approved for clinical diagnosis or patient care. Developed by ImCare Biotech, Seravue® supports hepatocellular carcinoma (HCC) detection, including early-stage identification and monitoring of at-risk individuals.
| Component | Amount |
| 96-strip well microplate | 96 (8x12) wells |
| 10X Calibrators A-G (10, 5, 2.5, 1.25, 0.62, 0.31, 0 ng/mL) | 7 vials, 100 µL |
|
Positive control (10X) – native human LC-SPIK in media |
100 µL |
| Negative control (10X) - media without LC-SPIK | 100 µL |
| 1X Dilution Buffer contains PBS, BSA | 25 mL |
| 100X Detection antibody - Enzyme conjugate, HRP labeled | 110 µL |
| TMB Solution | 11 mL |
| Stop solution, contains acid | 11 mL |
| 20X Wash Buffer - contains detergent | 2 bottles, 50 mL |
Human LC-SPIK ELISA Kit Summary
| Assay Length | ~ 2 hours |
| Sample Type | Human Serum, Human Plasma (EDTA) and Cellular Media |
| Sample/Assay Volume | Sample volume 10 µL , Assay well volume 100 µL |
| Sensitivity | ~ 0.05 ng/mL |
| Assay Range | 0.31 - 10.0 ng/mL (Cellular media, Plasma and Serum) |
| Specificity | Native and Recombinant Human LC-SPIK |
| Interference | No significant Interference observed from common interferants - avoid using grossly hemolyzed or lipemic samples |
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