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ImCare Biotech, Inc
Human AFP ELISA Kit (RUO)
Human AFP ELISA Kit (RUO)
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$499.00 USD
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$499.00 USD
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AFP ELISA Kit is a sensitive enzyme-linked immunosorbent assay designed for the precise quantification of human AFP levels in serum and plasma samples. It enables accurate in vitro measurement of Alpha-fetoprotein (AFP) levels in human biological fluids. This investigational use only (IUO) kit is intended for product evaluation and performance assessment in detecting AFP concentrations but is not approved for clinical diagnosis or patient care. Developed by ImCare Biotech, AFP ELISA kit supports hepatocellular carcinoma (HCC) detection.
| Component | Amount |
| 96-strip well microplate | 96 (8x12) wells |
| Human AFP Standard (160 ng/mL) | 2 vials, 200 µL each |
| 1X Dilution Buffer contains PBS, BSA | 25 mL |
| 100X Detection antibody - Enzyme conjugate, HRP labeled | 150 µL |
| TMB Solution | 11 mL |
| Stop solution, contains acid | 11 mL |
| 20X Wash Buffer - contains detergent | 2 bottles, 50 mL |
Human AFP ELISA Kit Summary
| Assay Length | ~ 2 hours |
| Sample Type | Human Serum, Human Plasma (EDTA) and Cellular Media |
| Sample/Assay Volume | Sample volume 10 µL , Assay well volume 100 µL |
| Sensitivity | ~ 0.05 ng/mL |
| Assay Range | 0.25 - 16.0 ng/mL (Cellular media, Plasma and Serum) |
| Specificity | Native and Recombinant Human AFP |
| Interference | No significant Interference observed from common interferants - avoid using grossly hemolyzed or lipemic samples |
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